Medial calcification in the human aorta accumulates throughout ageing and is understood to be aggravated in a number of ailments. Atherosclerosis, one other main explanation for cardiovascular calcification, shares some frequent aggravators. Nonetheless, the mechanisms of cardiovascular calcification stay poorly understood. To elucidate the relationship between medial aortic calcification and atherosclerosis, we characterised the cross-sectional distributions of the predominant minerals in aortic tissue, apatite and whitlockite, and the related extracellular matrix. We additionally in contrast the mobile adjustments between atherosclerotic and nonatherosclerotic human aortic tissues. This was achieved by means of the improvement of Raman spectroscopy imaging strategies that tailored algorithms to differentiate between the main biomolecules current inside these tissues. We current a relationship between apatite, ldl cholesterol, and triglyceride in atherosclerosis, with the relative quantity of all molecules concurrently elevated in the atherosclerotic plaque. Additional, the improve in apatite was disproportionately giant in relation to whitlockite in the aortic media straight underlying a plaque, indicating that apatite is extra pathologically important in atherosclerosis-aggravated medial calcification. We additionally found a discount of β-carotene in the entire aortic intima, together with a plaque in atherosclerotic aortic tissues in comparison with nonatherosclerotic tissues. This unprecedented biomolecular characterization of the aortic tissue furthers our understanding of pathological and physiological cardiovascular calcification occasions in people.